Journal: Frontiers in Aging Neuroscience

Article Title: Alix: A Candidate Serum Biomarker of Alzheimer’s Disease

doi: 10.3389/fnagi.2021.669612

Figure Lengend Snippet: (A) Western blot analysis of Alix in the cortex tissues from Alzheimer’s disease (AD) patients and controls. (B) Western blot analysis of Alix in the hippocampus tissues from AD patients and controls. (C) Western blot analysis of Alix in serum samples from AD patients and controls. Quantified results were normalized to β-actin/IgG expression. Values were expressed as percentages compared to the controls (set to 100%) and represented as means ± SEM. n = 12 per group. * p < 0.05, ** p < 0.01 vs. the control group, Student’s t -test.

Article Snippet: Alix levels in serum were measured using a commercially available human Alix quantitative sandwich enzyme immunoassay (Uscnk, Wuhan, China).

Techniques: Western Blot, Expressing, Control

Journal: Frontiers in Aging Neuroscience

Article Title: Alix: A Candidate Serum Biomarker of Alzheimer’s Disease

doi: 10.3389/fnagi.2021.669612

Figure Lengend Snippet: Alix levels in serum are presented as box plots for AD patients and healthy controls. The lower and upper sides of the boxes indicate the 25th and 75th percentiles, and the horizontal lines indicate the means. The lower and upper whiskers are also shown that indicate the minimum and maximum values, respectively. n = 404 per group. ** p < 0.01 vs. the control group, Student’s t -test.

Article Snippet: Alix levels in serum were measured using a commercially available human Alix quantitative sandwich enzyme immunoassay (Uscnk, Wuhan, China).

Techniques: Control

Journal: Frontiers in Aging Neuroscience

Article Title: Alix: A Candidate Serum Biomarker of Alzheimer’s Disease

doi: 10.3389/fnagi.2021.669612

Figure Lengend Snippet: Alix and Aβ 40 levels in serum of AD patients and Controls.

Article Snippet: Alix levels in serum were measured using a commercially available human Alix quantitative sandwich enzyme immunoassay (Uscnk, Wuhan, China).

Techniques:

Journal: Frontiers in Aging Neuroscience

Article Title: Alix: A Candidate Serum Biomarker of Alzheimer’s Disease

doi: 10.3389/fnagi.2021.669612

Figure Lengend Snippet: (A) Correlation analysis between Alix serum levels and MMSE scores in AD patients. (B) Correlation analysis between Alix and Aβ 40 in serum levels. Correlation was assessed using the Spearman correlation coefficient. A significant positive correlation between serum Alix level and MMSE score ( r = 0.80, p < 0.001) was observed, as well as a significant negative correlation between Alix and Aβ 40 in serum levels ( r = −0.60, p < 0.001). Correlation lines are also shown.

Article Snippet: Alix levels in serum were measured using a commercially available human Alix quantitative sandwich enzyme immunoassay (Uscnk, Wuhan, China).

Techniques:

Journal: Frontiers in Aging Neuroscience

Article Title: Alix: A Candidate Serum Biomarker of Alzheimer’s Disease

doi: 10.3389/fnagi.2021.669612

Figure Lengend Snippet: (A) ROC curve analysis for serum Alix concentration and the prediction of the presence of AD. The AUC was 0.80. The optimal cut-off value (199.5 pg/ml) was selected. The diagnostic accuracy for Aix protein levels was 74% with the sensitivity and specificity 76% and 71%, respectively. (B) ROC curve analysis for the combination of serum Aβ 40 and Alix. The AUC value of Aβ 40 was 0.69, and the AUC for the combined detection of Aβ 40 and Alix was raised to 0.75. Abbreviations: ROC, receiver operating characteristic; AUC, area under the curve.

Article Snippet: Alix levels in serum were measured using a commercially available human Alix quantitative sandwich enzyme immunoassay (Uscnk, Wuhan, China).

Techniques: Concentration Assay, Diagnostic Assay

Journal: Frontiers in Aging Neuroscience

Article Title: Alix: A Candidate Serum Biomarker of Alzheimer’s Disease

doi: 10.3389/fnagi.2021.669612

Figure Lengend Snippet: (A) Western blot analysis of Alix in brain tissues from AD patients, vascular dementia (VaD) patients and the controls. Equal volume of each sample was loaded and β-actin was used as a loading control. Values were expressed as percentages compared to control group (set to 100%), and represented as means ± SEM. n = 12 for AD and control group, and n = 8 for VaD group. ** p < 0.01 vs. the control group, # p < 0.05 vs. AD group. (B) Scatter plots of Alix levels in serum of VaD ( n = 52), AD ( n = 404) and Controls ( n = 404) measured by ELISA. ** p < 0.01. (C) ROC curve analysis for serum Alix between VaD and AD. The AUC was 0.777.

Article Snippet: Alix levels in serum were measured using a commercially available human Alix quantitative sandwich enzyme immunoassay (Uscnk, Wuhan, China).

Techniques: Western Blot, Control, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Aging Neuroscience

Article Title: Alix: A Candidate Serum Biomarker of Alzheimer’s Disease

doi: 10.3389/fnagi.2021.669612

Figure Lengend Snippet: (A) Western blot analysis of Alix after the treatment of Memantine in serum samples from APP/PS1 mice and wild-type (WT) mice. Quantified results were normalized to IgG expression. Values were expressed as percentages compared to WT group (set to 100%), and represented as means ± SEM. n = 8–10. ** p < 0.01 vs. the WT group, # p < 0.05 vs. APP/PS1 mice. (B) Western blot analysis of Alix after the treatment of Memantine in serum samples from Aβ 25–35 intracerebroventricular-injected rats and age-matched controls. Values were expressed as percentages compared to the control group (set to 100%), and represented as means ± SEM. n = 8–10. ** p < 0.01 vs. the control group, # p < 0.05 vs. Aβ 25–35 intracerebroventricular-injected rats.

Article Snippet: Alix levels in serum were measured using a commercially available human Alix quantitative sandwich enzyme immunoassay (Uscnk, Wuhan, China).

Techniques: Western Blot, Expressing, Injection, Control